Project #1022

Overcoming Molecular Sample Processing Limitations

$0
Completed
Principal Investigator
James
Higgins
Research Manager
Dr. Daniel M. Woltering, Ph.D.
Contractor
USDA Agriculture Research Service
Microbes & Pathogens
Monitoring

Abstract

Molecular methods appear to be a promising way to rapidly detect pathogens in water. However, researchers need to identify and evaluate sample preparation techniques that are high-throughput and cost-effective, require minimum training for use, and are reproducible from user to user. This three-part volume covers important topics in molecular sampling: RNA and DNA extraction strategies (James A. Higgins), quantitative polymerase chain reaction (George D. Di Giovanni, PhD), and fiber-optic biosensors (Valerie J. Harwood, PhD). Published in 2002.

Originally funded as WERF project 00-HHE-2a, 00-HHE-2b, and 00-HHE-2c.

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