Overcoming Molecular Sample Processing Limitations

Abstract

Molecular methods appear to be a promising way to rapidly detect pathogens in water. However, researchers need to identify and evaluate sample preparation techniques that are high-throughput and cost-effective, require minimum training for use, and are reproducible from user to user. This three-part volume covers important topics in molecular sampling: RNA and DNA extraction strategies (James A. Higgins), quantitative polymerase chain reaction (George D. Di Giovanni, PhD), and fiber-optic biosensors (Valerie J. Harwood, PhD). Published in 2002.

Originally funded as WERF project 00-HHE-2a, 00-HHE-2b, and 00-HHE-2c.

Resources

Public Plus

Overcoming Molecular Sample Processing Limitations

Report

#0HHE2

04/18/2002

View Executive Summary

Related Projects

Recently

Project #5104

Use of DNA Nanostructures as Viral Surrogates in Potable Reuse Applications

Completed

This research developed and validated novel surrogates for awarding virus log reduction values (LRVs) in membrane-based potable reuse systems. Specifically, the project involves three research themes: (1) development...

View more details

Project #1067

Molecular Alternatives to Indicator and Pathogen Detection: Real-Time PCR

Completed

Molecular methods appear to be a promising way to rapidly detect pathogens in water. However, researchers need to identify and evaluate sample preparation techniques that are high-throughput and...

View more details

Project #5093

Understanding the Factors That Affect the Detection and Variability of SARS-CoV-2 in Wastewater

Completed

Wastewater-based surveillance (WBS) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represents a promising complement to clinical testing as a means of assessing COVID-19 trends within a community...

View more details

Project #5029

Environmental Persistence and Disinfection of the Lassa Virus and SARS-CoV-2 to Protect Worker and Public Safety

In Progress

The Ebola virus epidemic of 2014-15 revealed significant shortcomings in the wastewater sector regarding response to highly infectious disease outbreaks. The overarching goal of this research was to...

View more details

Project #5126

Advancing Safety and Reliability to Protect Public Health: Identifying Quantitative Reductions of Viral Pathogens and Surrogates for Water Reuse Applications

In Progress

This project will identify quantitative log reductions of viral pathogens and appropriate surrogates across unit treatment processes and full treatment trains for water reuse applications. This will include...

View more details

Project #5100

Rapid Detection and Quantitation of Active Microorganisms

In Progress

The detection and rapid quantitation of active microorganisms in complex microbial communities are important for informing process monitoring and control and risk assessment in the water sector. However...

View more details

Wastewater Process Emissions Fundamentals

Overcoming Molecular Sample Processing Limitations

Abstract

Resources

Overcoming Molecular Sample Processing Limitations

Use of DNA Nanostructures as Viral Surrogates in Potable Reuse Applications

Molecular Alternatives to Indicator and Pathogen Detection: Real-Time PCR

Understanding the Factors That Affect the Detection and Variability of SARS-CoV-2 in Wastewater

Environmental Persistence and Disinfection of the Lassa Virus and SARS-CoV-2 to Protect Worker and Public Safety

Advancing Safety and Reliability to Protect Public Health: Identifying Quantitative Reductions of Viral Pathogens and Surrogates for Water Reuse Applications

Rapid Detection and Quantitation of Active Microorganisms

Wastewater Process Emissions Fundamentals